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pcag egxxfp plasmid  (Addgene inc)


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    Structured Review

    Addgene inc pcag egxxfp plasmid
    Pcag Egxxfp Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcag+egxxfp+plasmid/pCAG-EGxxFP+(Plasmid+%2350716)/us12590323-385-41-43
    Average 93 stars, based on 100 article reviews
    pcag egxxfp plasmid - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Genome Editing in Mice Using CRISPR/Cas9 Technology
    Article Snippet: .. The efficiency of sgRNA can also be readily examined using the pCAG-EGXXFP plasmid (available at Addgene), which employs a fluorescent means to determine if a DSB is generated ( Mashiko et al., 2013 ). ..

    Article Title: Functional analysis of O-GlcNAcylation by networking of OGT interactors and substrates.
    Article Snippet: The genomic C-terminal region of Ogt was amplified using the Q5 HotStart high-fidelity 2× master mix (New England Biolabs). .. The pCAG-EGxxFP plasmid (Addgene, 50716; M. Ikawa) was cut with BamHI and SalI and the construct was assembled using the NEBuilder HiFi assembly kit. sgRNA candidates were cloned into the pX330 plasmid (Addgene, 42230; F. Zhang) according to the published protocol. ..

    Article Title: Functional glycoproteomics by integrated network assembly and partitioning
    Article Snippet: The genomic C-terminal region of OGT was amplified using the Q5 Hot Start High Fidelity 2x master mix (New England Biolabs). .. The pCAG-EGxxFP plasmid (Addgene, Masahito Ikawa; 50716) was cut with BamHI and SalI, and the construct was assembled using the NEBuilder HiFi Assembly kit. sgRNA candidates were cloned into the pX330 plasmid (Addgene, Feng Zhang; 42230) according to the published protocol. ..

    Article Title: Computationally defined and in vitro validated putative genomic safe harbour loci for transgene expression in human cells
    Article Snippet: .. The amplified target sequences, ranging from 232 to 974 bp were cloned into the pCAG-EGxxFP plasmid ( ) (Addgene # 50716, a kind gift from Dr Masahito Ikawa). .. The pMIA3 with the tested gRNA and the respective pCAG-EGxxFP target plasmid were transfected into HEK293T using lipofectamine3000 (Thermo Fisher Scientific, L3000015), according to manufacturer’s recommendations.

    Article Title: Compositions for use in treating autosomal dominant BEST1-related retinopathies
    Article Snippet: .. To evaluate computationally designed sgRNAs for efficiency and allele specificity, we adapted a fluorescence-based assay and cloned a genomic fragment of ̃500 bp containing either the on-target or the non-target BEST1 sequence between EGFP (enhanced green fluorescent protein) fragments of the pCAG-EGxxFP plasmid (Addgene, UK). .. The resulting target plasmid was co-transfected into HEK293 cells with the SpCas9-expressing plasmid pU6-(BbsI)_CBh-Cas9-T2A-mCherry (Addgene) and the corresponding sgRNA with varying sequence length (20-17 bp).

    Generated:

    Article Title: Genome Editing in Mice Using CRISPR/Cas9 Technology
    Article Snippet: .. The efficiency of sgRNA can also be readily examined using the pCAG-EGXXFP plasmid (available at Addgene), which employs a fluorescent means to determine if a DSB is generated ( Mashiko et al., 2013 ). ..

    Construct:

    Article Title: Functional analysis of O-GlcNAcylation by networking of OGT interactors and substrates.
    Article Snippet: The genomic C-terminal region of Ogt was amplified using the Q5 HotStart high-fidelity 2× master mix (New England Biolabs). .. The pCAG-EGxxFP plasmid (Addgene, 50716; M. Ikawa) was cut with BamHI and SalI and the construct was assembled using the NEBuilder HiFi assembly kit. sgRNA candidates were cloned into the pX330 plasmid (Addgene, 42230; F. Zhang) according to the published protocol. ..

    Article Title: Functional glycoproteomics by integrated network assembly and partitioning
    Article Snippet: The genomic C-terminal region of OGT was amplified using the Q5 Hot Start High Fidelity 2x master mix (New England Biolabs). .. The pCAG-EGxxFP plasmid (Addgene, Masahito Ikawa; 50716) was cut with BamHI and SalI, and the construct was assembled using the NEBuilder HiFi Assembly kit. sgRNA candidates were cloned into the pX330 plasmid (Addgene, Feng Zhang; 42230) according to the published protocol. ..

    Clone Assay:

    Article Title: Functional analysis of O-GlcNAcylation by networking of OGT interactors and substrates.
    Article Snippet: The genomic C-terminal region of Ogt was amplified using the Q5 HotStart high-fidelity 2× master mix (New England Biolabs). .. The pCAG-EGxxFP plasmid (Addgene, 50716; M. Ikawa) was cut with BamHI and SalI and the construct was assembled using the NEBuilder HiFi assembly kit. sgRNA candidates were cloned into the pX330 plasmid (Addgene, 42230; F. Zhang) according to the published protocol. ..

    Article Title: Functional glycoproteomics by integrated network assembly and partitioning
    Article Snippet: The genomic C-terminal region of OGT was amplified using the Q5 Hot Start High Fidelity 2x master mix (New England Biolabs). .. The pCAG-EGxxFP plasmid (Addgene, Masahito Ikawa; 50716) was cut with BamHI and SalI, and the construct was assembled using the NEBuilder HiFi Assembly kit. sgRNA candidates were cloned into the pX330 plasmid (Addgene, Feng Zhang; 42230) according to the published protocol. ..

    Article Title: Computationally defined and in vitro validated putative genomic safe harbour loci for transgene expression in human cells
    Article Snippet: .. The amplified target sequences, ranging from 232 to 974 bp were cloned into the pCAG-EGxxFP plasmid ( ) (Addgene # 50716, a kind gift from Dr Masahito Ikawa). .. The pMIA3 with the tested gRNA and the respective pCAG-EGxxFP target plasmid were transfected into HEK293T using lipofectamine3000 (Thermo Fisher Scientific, L3000015), according to manufacturer’s recommendations.

    Article Title: Compositions for use in treating autosomal dominant BEST1-related retinopathies
    Article Snippet: .. To evaluate computationally designed sgRNAs for efficiency and allele specificity, we adapted a fluorescence-based assay and cloned a genomic fragment of ̃500 bp containing either the on-target or the non-target BEST1 sequence between EGFP (enhanced green fluorescent protein) fragments of the pCAG-EGxxFP plasmid (Addgene, UK). .. The resulting target plasmid was co-transfected into HEK293 cells with the SpCas9-expressing plasmid pU6-(BbsI)_CBh-Cas9-T2A-mCherry (Addgene) and the corresponding sgRNA with varying sequence length (20-17 bp).

    Amplification:

    Article Title: Computationally defined and in vitro validated putative genomic safe harbour loci for transgene expression in human cells
    Article Snippet: .. The amplified target sequences, ranging from 232 to 974 bp were cloned into the pCAG-EGxxFP plasmid ( ) (Addgene # 50716, a kind gift from Dr Masahito Ikawa). .. The pMIA3 with the tested gRNA and the respective pCAG-EGxxFP target plasmid were transfected into HEK293T using lipofectamine3000 (Thermo Fisher Scientific, L3000015), according to manufacturer’s recommendations.

    Fluorescence:

    Article Title: Compositions for use in treating autosomal dominant BEST1-related retinopathies
    Article Snippet: .. To evaluate computationally designed sgRNAs for efficiency and allele specificity, we adapted a fluorescence-based assay and cloned a genomic fragment of ̃500 bp containing either the on-target or the non-target BEST1 sequence between EGFP (enhanced green fluorescent protein) fragments of the pCAG-EGxxFP plasmid (Addgene, UK). .. The resulting target plasmid was co-transfected into HEK293 cells with the SpCas9-expressing plasmid pU6-(BbsI)_CBh-Cas9-T2A-mCherry (Addgene) and the corresponding sgRNA with varying sequence length (20-17 bp).

    Sequencing:

    Article Title: Compositions for use in treating autosomal dominant BEST1-related retinopathies
    Article Snippet: .. To evaluate computationally designed sgRNAs for efficiency and allele specificity, we adapted a fluorescence-based assay and cloned a genomic fragment of ̃500 bp containing either the on-target or the non-target BEST1 sequence between EGFP (enhanced green fluorescent protein) fragments of the pCAG-EGxxFP plasmid (Addgene, UK). .. The resulting target plasmid was co-transfected into HEK293 cells with the SpCas9-expressing plasmid pU6-(BbsI)_CBh-Cas9-T2A-mCherry (Addgene) and the corresponding sgRNA with varying sequence length (20-17 bp).



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    Addgene inc pcag egxxfp egfp expression plasmid
    Schematic diagram of survivin gene knockout using clustered regularly interspaced short palindromic repeats (CRISPR)-associated nuclease 9 (CRISPR/Cas9) in triple-negative breast cancer (TNBC) cell line BT549. <t>EGFP:</t> enhanced green fluorescent protein; PCR: polymerase chain reaction; sgRNA: single guide RNA
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    Image Search Results


    Schematic diagram of survivin gene knockout using clustered regularly interspaced short palindromic repeats (CRISPR)-associated nuclease 9 (CRISPR/Cas9) in triple-negative breast cancer (TNBC) cell line BT549. EGFP: enhanced green fluorescent protein; PCR: polymerase chain reaction; sgRNA: single guide RNA

    Journal: Narra J

    Article Title: Dual sgRNA-directed knockout survivin gene expression using CRISPR/Cas9 technology for editing survivin gene in triple-negative breast cancer

    doi: 10.52225/narra.v4i3.1177

    Figure Lengend Snippet: Schematic diagram of survivin gene knockout using clustered regularly interspaced short palindromic repeats (CRISPR)-associated nuclease 9 (CRISPR/Cas9) in triple-negative breast cancer (TNBC) cell line BT549. EGFP: enhanced green fluorescent protein; PCR: polymerase chain reaction; sgRNA: single guide RNA

    Article Snippet: The cleavage activity of each sgRNA was assessed using an enhanced green fluorescent protein (EGFP) system, as previously described [ ]. sgRNA target sequences were amplified by PCR and inserted into the pCAG-EGxxFP EGFP expression plasmid (Plasmid #50716, Addgene, Watertown, Massachusetts, USA) [ ], at the BamHI and EcoRI restriction sites.

    Techniques: Gene Knockout, CRISPR, Polymerase Chain Reaction